Journal: Antioxidants

Article Title: A Preliminary Study on the Effect of Hydrogen Gas on Alleviating Early CCl 4 -Induced Chronic Liver Injury in Rats

doi: 10.3390/antiox10121933

Figure Lengend Snippet: Changes in the expression of uncoupling protein 2 (UCP2) were observed by immunohistochemistry. ( a , b ) The expression of UCP2 in liver tissue of control group rats. A small number of UCP2-positive cells were brownish yellow in the cytoplasm. ( c , d ) The expression of UCP2 in liver tissue of CCl 4 group rats. A small number of UCP2 positive cells were brownish yellow in the cytoplasm. ( e , f ) The expression of UCP2 in liver tissue of CCl 4 + H 2 group rats. UCP2 was diffusely expressed in liver cells, and the staining sites were mostly located on the cell membrane. ( g , h ) The expression of UCP2 in liver tissue of H 2 group rats. The number of UCP2 positive cells with brownish-yellow staining in the cytoplasm increased significantly. ( a , c , e , g ) Fields of vision at 40× magnification. ( b , d , f , h ) Higher magnifications of the area outlined in ( a , c , e , g ). The long scale bar refers to 100 μm. The short scale bar refers to 50 μm.

Article Snippet: The membrane was incubated with antibodies of goat anti-UCP2 (bs-20750R, Bioss, Beijing, China) and mouse anti-β actin (A2228, Sigma, St. Louis, MO, USA) overnight at 4 °C.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Antioxidants

Article Title: A Preliminary Study on the Effect of Hydrogen Gas on Alleviating Early CCl 4 -Induced Chronic Liver Injury in Rats

doi: 10.3390/antiox10121933

Figure Lengend Snippet: UCP2-positive area rate. N = 8, * p < 0.05.

Article Snippet: The membrane was incubated with antibodies of goat anti-UCP2 (bs-20750R, Bioss, Beijing, China) and mouse anti-β actin (A2228, Sigma, St. Louis, MO, USA) overnight at 4 °C.

Techniques:

Journal: Antioxidants

Article Title: A Preliminary Study on the Effect of Hydrogen Gas on Alleviating Early CCl 4 -Induced Chronic Liver Injury in Rats

doi: 10.3390/antiox10121933

Figure Lengend Snippet: Expression changes of UCP2 (Western blotting). ( a ) Representative western blotting image of UCP2. ( b ) Statistical results of western blotting. N = 8, * p < 0.05.

Article Snippet: The membrane was incubated with antibodies of goat anti-UCP2 (bs-20750R, Bioss, Beijing, China) and mouse anti-β actin (A2228, Sigma, St. Louis, MO, USA) overnight at 4 °C.

Techniques: Expressing, Western Blot

Journal: BMC Physiology

Article Title: Both ischemic preconditioning and ghrelin administration protect hippocampus from ischemia/reperfusion and upregulate uncoupling protein-2

doi: 10.1186/1472-6793-9-17

Figure Lengend Snippet: Expression of UCP-2 in CA1 area of hippocampus (confocal microscope images of UCP2 staining by indirect immunohistochemistry) as observed with sc6525 (A, B, C, D) anti-UCP2 (C-terminal antibody) . A, control; B, I/R; C, IPC+I/R; D, IPC. NC, negative control obtained in the absence of the anti-UCP2. Scale bar = 30 μm. E: Bar graph of results of UCP-2 immunofluorescence (sc6525 images) in the CA1 pyramidal layer, expressed as average intensity after subtraction of background fluorescence (mean ± S.E.M, n = 4 animals). Significant differences were determined by repeated measures ANOVA plus Tukey's test. * P < 0.05 compared to all other groups.

Article Snippet: The primary antibodies used were: 1) goat anti-UCP2 C-terminus (sc-6525), or goat anti-UCP2 N-terminus (sc-6526) both from Santa Cruz Biotechnology, diluted ×200; 2) mouse anti-NeuN (MAB377, Chemicon), diluted ×250.

Techniques: Expressing, Microscopy, Staining, Immunohistochemistry, Negative Control, Immunofluorescence, Fluorescence

Journal: BMC Physiology

Article Title: Both ischemic preconditioning and ghrelin administration protect hippocampus from ischemia/reperfusion and upregulate uncoupling protein-2

doi: 10.1186/1472-6793-9-17

Figure Lengend Snippet: Control experiments . a, b, c, d, expression of UCP-2 in CA1 area of hippocampus (confocal microscope images of UCP2 staining by indirect immunohistochemistry) in examples of the 4 groups indicated, as observed with sc6526 (N-terminal antibody). A, B, C, indirect immunohistochemical labeling of UCP2 (red) in stomach (A, B, C). The DNA is stained blue with DAPI. White arrows in A and B indicate some of the UCP2-positive cells of the mucosal layer. Typically, there are more in the stomach from a fasted animal (B) than from that of a control animal (A). C shows detail of one cell, with an overlapping zone (pink) of UCP2 immunoreactivity and DAPI. D1, D2, CA1 neurons of the hippocampus showing pyramidal cells from a control animal and an IPC animal respectively. Notice the proximal dendritic staining of UCP2 in the highly stained IPC animal (arrows in D2). E, F, high power fields of pyramidal cells in an IPC animal, E with UCP2 staining and F without UCP2 staining. Notice the difference in the apparent shape and size of pyramidal cell nuclei between control (D1, round unstained nuclei) and IPC animals (F, polygonal DAPI-stained nuclei, red arrows). Widespread homogeneous UCP2 immunoreactivity was present in the pyramidal cell cytoplasm in the IPC group (E, star); it also appeared interspersed, non-homogeneously, with the DAPI staining. UCP2 staining with sc6525 except in D (sc6526).

Article Snippet: The primary antibodies used were: 1) goat anti-UCP2 C-terminus (sc-6525), or goat anti-UCP2 N-terminus (sc-6526) both from Santa Cruz Biotechnology, diluted ×200; 2) mouse anti-NeuN (MAB377, Chemicon), diluted ×250.

Techniques: Expressing, Microscopy, Staining, Immunohistochemistry, Immunohistochemical staining, Labeling

Journal: BMC Physiology

Article Title: Both ischemic preconditioning and ghrelin administration protect hippocampus from ischemia/reperfusion and upregulate uncoupling protein-2

doi: 10.1186/1472-6793-9-17

Figure Lengend Snippet: Effects of SOD treatment on UCP2 immunoreactivity (indirect method) in the hippocampal CA1 area . Confocal microscope images of UCP2 immunoreactivity of A: IPC group; B: IPC + SOD group; C: IPC +vehicle group. Notice the similarity in the appearance of SOD-treated neurons in B with that of control neurons in Fig 2A. D: Bar graph of fluorescence intensities of UCP-2 staining (mean ± S.E.M, n = 4 animals). Scale bar A, B, C = 15 μm. Significant differences were determined by repeated measures ANOVA plus Tukey's test. *** P < 0.001 compared to IPC group; ## P < 0.01 compared to IPC + SOD group.

Article Snippet: The primary antibodies used were: 1) goat anti-UCP2 C-terminus (sc-6525), or goat anti-UCP2 N-terminus (sc-6526) both from Santa Cruz Biotechnology, diluted ×200; 2) mouse anti-NeuN (MAB377, Chemicon), diluted ×250.

Techniques: Microscopy, Fluorescence, Staining

Journal: BMC Physiology

Article Title: Both ischemic preconditioning and ghrelin administration protect hippocampus from ischemia/reperfusion and upregulate uncoupling protein-2

doi: 10.1186/1472-6793-9-17

Figure Lengend Snippet: Effects of post-conditioning ischemia time on UCP2 immunoreactivity in the hippocampal CA1 area . Confocal microscope images of UCP2 immunoreactivity of A: control group; B: D3 group (3 days post-ischemia); C: D6 group (6 days post-ischemia). D: Bar graph of fluorescence intensities of UCP2 staining (mean ± SEM, n = 4 animals). Significant differences were determined by repeated measures ANOVA plus Tukey's test. ** P < 0.05 compared to Control or D6. Scale bar E, F, G = 50 μm.

Article Snippet: The primary antibodies used were: 1) goat anti-UCP2 C-terminus (sc-6525), or goat anti-UCP2 N-terminus (sc-6526) both from Santa Cruz Biotechnology, diluted ×200; 2) mouse anti-NeuN (MAB377, Chemicon), diluted ×250.

Techniques: Microscopy, Fluorescence, Staining

Journal: BMC Physiology

Article Title: Both ischemic preconditioning and ghrelin administration protect hippocampus from ischemia/reperfusion and upregulate uncoupling protein-2

doi: 10.1186/1472-6793-9-17

Figure Lengend Snippet: Effects of various treatments on UCP-2 mRNA expression in ghrelin experiments . RT-PCR of UCP2 mRNA in the hippocampal preparations in the control group, I/R group (vehicle alone), or I/R group treated with ghrelin (G). A, B: Typical bands obtained for, respectively, β-actin and UCP-2 mRNA. C: Quantitative results expressed with respect to β-actin mRNA in each of the three groups. n = 6 animals. *** significantly different from the control and I/R groups, P < 0.001. # significantly different from the control group, P < 0.05. ANOVA + Tukey's test.

Article Snippet: The primary antibodies used were: 1) goat anti-UCP2 C-terminus (sc-6525), or goat anti-UCP2 N-terminus (sc-6526) both from Santa Cruz Biotechnology, diluted ×200; 2) mouse anti-NeuN (MAB377, Chemicon), diluted ×250.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction